There are several sophisticated analytical methods that are used most abundantly for the precise quantitative methods microbial assays, such as :
(a) High Performance Liquid Chromatography (HPLC),
(b) Reverse-Phase Chromatography (RPC), and
(c) Ion–Pair (or Paired-Ion) Chromatography,

These three chromatographic techniques shall now be discussed briefly in the sections that follows:
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Continuing last articles about Rapid-Reliable-Reproducible Microbial Assay Methods, its time to discuss about Lucifrerase Assay.

In the specific ‘Luciferase Assay’, the firefly luciferase** is made use of for the actual measurement of small quantum of ATP duly present in a microbial culture, whereby the levels of ATP get proportionately reduced by the ensuing action of the aminoglycoside antibiotics.
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In a broader perspective the wide-spectrum of the pharmaceutical products, both pure and dosage forms, may be accomplished by adopting any one of the following two well-recognized, time tested, and universally accepted methods, namely :
(a) Membrane Filtration, and
(b) Direct Inoculation.
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Application of pharmaceutical principles to drug dosage forms is illust rated when drug dosage forms are categorized according to their physical state, degree of heterogeneity, and chemical composition. The usual relevant states of matter are gases, liquids, and solids. Intermolecular forces of attraction are weakest in gases and strongest in solids. Conversions from one physical state to another can involve simply overcoming intermolecular forces of attraction by adding energy (heat) . Chemical composition can have a dramatic effect on physicochemical properties and behavior. For this reason, it is necessary to distinguish between polymers, or macromolecules, and more conventional ( i .e., smaller) molecules, or micromolecules.
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